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KMID : 0357319940290010001
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Journal of the Korean Society for Microbiology
1994 Volume.29 No. 1 p.1 ~ p.26
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The Relationships between the Phage, Pyocin Typing and the Resistant Plasmid to Antimicrobial Agents of Pseudomonas aeruginosa
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Abstract
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Pseudomonas aeruginosa can be readily isolated from the natural environment and man, and it is not a homogenous species. Numerous methods have been proposed for the identification of strains within the species and some have been adopted
universally.
Current knowledge of typing Pseudomonas aeruginosa and new methods for characterizing strains are reviewed. A combination of serotyping as a primary screen with pyocin typing for finer discrimination between isolates gives valid epidemiolgical
data, is
within the scope of most clinical laboratories and is to be recommended. In this study, eighty strains of Pseudomonas aeruginosa from urine, sputum, stool, pus and others in out-patients of Pusan National University were test for antimicrobial
susceptibility patterns, synergistic activity test, plasmid DNA profile, biotyping, seroligical typing phage typing and pyocin typing. The results of this study demonstrate that the electrophoretic patterns of plasmid DNA profile in combination
with
antimicrobial susceptibility patterns, synergistic activity test or biotyping, serological typing and phage typing, pyocin typing, can be of value in the epidemiologic fingerprinting of clinical isolates of Pseudomonas aeruginosa.
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KEYWORD
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